If you notice that your audio playback is out of tune despite correct settings, please make sure that the "global detune" button has not been changed accidentally.
If your playback is still out of tune and does not reflect your tuning settings:
- Open your Finder
- Go to "Applications" and select "Utilities"
- Select "Audio MIDI Setup"
- Make sure that your rate format is set to 44100,0 Hz.
- Correct it accordingly if necessary.